BioFlo Workbench
🚀 Open 3D Bioreactor CAD 📊 Cell Culture Numbers

Transfection Reagent & DNA Ratio Calculator

Calculate precise plasmid DNA mass, transfection reagent volumes (PEI MAX, Lipofectamine, FuGENE), and Opti-MEM complexation media across multi-well plates, culture dishes, and large-scale suspension vessels.

Transfection Parameters Direct Setup
Master Mix Preparation Ready to Pipette
Total Plasmid DNA
15.0 μg
DNA Stock Volume
15.0 μL
Total Transfection Reagent
45.0 μL
Opti-MEM Diluent
1.50 mL
Complexation Checklist
Tube A (DNA Dilution): Add 15.0 μL plasmid DNA into 750 μL Opti-MEM. Vortex gently.
Tube B (Reagent Dilution): Dilute 45.0 μL reagent into 750 μL Opti-MEM. Mix gently.
Complexation: Combine Tube A and B (1:1), incubate for 15–20 min at room temperature, then add dropwise to culture vessels.

Scaling Up Viral Vector Transfection in Stirred Bioreactors?

Avoid shear-induced complex disruption. Simulate hydrodynamic shear rate, gas sparging, and oxygen transfer (OTR/OUR) in BioFlo Prajapati & Axiom.

Open Prajapati Bioprocess Engine ➔