Calculated maximum growth rate μ_max = 0.58 h⁻¹ and lag phase λ = 2.15 h ready to feed into Multi-Model Kinetic Parameter Fitting (Step 3) to resolve half-saturation $K_s$ and substrate inhibition $K_i$.
Step 2 of 5: Isolate exponential growth phase, calculate generation doubling time $t_d$, specific growth rate $\mu_{max}$, and fit Modified Gompertz equations. • 100% Free & Open Access.
Microbial batch cultures exhibit five characteristic phases: lag phase, exponential (log) growth phase, deceleration phase, stationary phase, and death phase. Accurately extracting specific growth rate ($\mu$, hr⁻¹) and generation doubling time ($t_d$, hr) during the true exponential phase is essential for metabolic flux analysis and seed train scheduling.
| Organism | Max Specific Growth Rate μ_max (hr⁻¹) | Doubling Time td (min) | Optimal Temperature (°C) |
|---|---|---|---|
| Escherichia coli | 0.60 – 1.20 | 20 – 70 min | 37.0°C |
| Bacillus subtilis | 0.50 – 0.90 | 45 – 80 min | 37.0°C |
| Saccharomyces cerevisiae | 0.35 – 0.45 | 90 – 120 min | 30.0°C |
| CHO Cell Culture | 0.025 – 0.040 | 18 – 28 hours | 36.5°C |
This calculator is maintained by the simulation engineers at BioFlo Bioprocess Engineering. We specialize in industrial bioreactor design, computational fluid dynamics (CFD) modeling, oxygen mass transfer optimization, and custom digital twin development for pharmaceutical fermentation plants.