BioFlo Bioprocess Workbench
Verified In-Silico Architecture

Fermentation Kinetics & Microbial Growth Simulators

Rigorous microbial kinetics modeling suite: Spectrophotometric OD600 calibration, Modified Gompertz growth dynamics, Monod/Haldane parameter estimation, yield balances, and Golden Batch SPC envelopes.

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100% Free Tool

Optical Density (OD600) to Biomass Converter

Calibrate spectrophotometer absorbance to cell dry weight (CDW g/L) and calculate required seed train inoculum volume.

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100% Free Tool

Growth Curve Dynamics & Doubling Time

Isolate exponential growth phase, calculate specific growth rate μ_max, generation doubling time td, and lag duration λ with Modified Gompertz fitting.

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100% Free Tool

Multi-Model Non-Linear Kinetic Fitting

Non-linear regression parameter estimation (Monod, Haldane, Hinshelwood, Moser, Tessier) from time-series OD data.

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100% Free Tool

Monod Yield Balances & Maintenance

Compute substrate-dependent specific growth rate μ(S), biomass yield (Yx/s), maintenance coefficient (ms), and chemostat washout limits.

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100% Free Tool

BatchCompare Golden Batch SPC Quality Envelopes

Benchmark multivariate fermentation runs against statistical golden batch envelopes (±1σ, ±2σ, ±3σ) with real-time anomaly alarms.

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100% Free Tool

Useful Cell Culture Numbers & Seeding Reference

Reference database for standard culture vessel surface areas, media working volumes, and target seeding densities.

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100% Free Tool

Transfection Reagent & DNA Ratio Calculator

Calculate required plasmid DNA mass and lipid/PEI reagent volumes across 6-well, 10cm, 15cm dishes and T-flasks.